Bulk RNA-seq in R
Carry one edgeR DGEList through filtering, normalization, differential expression, ranked enrichment, and sample scoring.
- Input
- Gene-level tximport counts and one metadata row per sample.
- Analysis unit
- Sample.
- Outputs
- edgeR contrasts, ranked enrichment, and sample-level signature scores.
- Key limit
- Replicates are samples; define pairing, batch, and other justified covariates in the model.